Agricultural Science

In Vitro Morphogenic Performance of Ginger (Zingiber Officinale) as Influenced by Media Variations

In Vitro Morphogenic Performance of Ginger (Zingiber Officinale) as Influenced by Media Variations

ABSTRACT

Ginger (Zingiber officinale Rose) is a perennial herb that grows from undergroundrhizomes. The demand for fresh and dry ginger and its essential oil in the world marketis high. Ginger is propagated vegetatively using underground rhizomes. Most farmersuse planting materials saved from previous harvest. These materials could have beensold for cash. Contingent on this, many farmers are reluctant to use healthy succulentrhizomes for planting. These are rather sold thereby resulting in acute shortage of planting materials.

Tissue culture technique can be applied to mass-produce seedlingsfor distribution to ginger farmers. This however, is not cost effective now due to lack ofthe necessary materials such as agar required for tissue culture. It is on the basis ofthese considerations that the present research was set up to develop a locally availableand cheap protocol that is reproducible in ginger plant tissue culture work with the following objectives:

To determine the best pre-initiation treatment for in-vitro gingermultiplication;

To determine the effect of plant growth hormones – auxin and cytokininon in-vitro propagation of ginger at the initiation stage using agar as a gelling agent;

To compare agar gelled medium with cassava gelled medium in in-vitro propagation ofginger at the initiation stage.

The research was carried out in the tissue culture researchlaboratory of the National Root Crop Research Institute (NRCRI), Umudike Station,Umuahia, Abia State. The explants were collected from ginger germplasm of the Institute.

References

Adaoha, M. E. N. and Roscoe, D. H. (1982). Some gelling agents for protoplast culture. Plant Science Letter, 2561.

Aharoni, N., Anderson, J. D. and Lieberman M. (1979). Production and action of ethylene in senescing leaf discs. Effects of indoleacetic acid, kinetin, silver ion, and carbon dioxide. Plant Physiology; 64:805-809.

Ahmed, M. F. (2002). Studies on the tissue culture and potential for the development of a genetic transformation system for avocados (Persea americana Mill.). Ph.D. Thesis, Univ. of Sydney, Australia.

Babu K. N., Samsudeen, K. and Ratnambal, M. J. (1992). In vitroplantregenaration from leafderived callus in ginger. Plant Cell Tiss. Org.Cult. 29: 71-74.

Bailey L. B. (1961) Manual of cultivated plants. The Macmillan Co., New York, pp 287-289.

Beltram I. C. and Kam Y. K. (1984). Cyto taxonomic studies in the Zingiberaceae. Notes from the Royal Bot. Garden, Edinburgh, 41: 541-557.

Bhagyalakshmi, A. and Singh, N. S. (1998). Meristem culture and micropropagation of a variety of ginger (Zingiber officinales Rosc.) with a high yield of oleoresin. .Journal of Hort.Sci. 321-327.

Bhat S. R., Chandel K. P. S. and Kackar A (1994). In vitro induction of rhizomes in ginger (Zingiber officinale Rosc.). Indian Journal of Experimental Biology, 32(5): 340-344.

Bonga J. M., Durzan D. J. (1985). Tissue Culture in Forest Trees. University of Califonia, Davis, U.S.A., 37-38.

Bushra Z., Nadeem A. A, Touqeer A. and Ishfaq A. H. (2009) Effect Of Explant Sources and Different Concentrations of Plant Growth Regulators On In Vitro Shoot Proliferation And Rooting Of Avocado (Persea Americana Mill.) Cv. “Fuerte”.Pak. J. Bot., 41(5): 2333-2346.

Chan L. K. and Thong W. H. (2004). In vitro propagation of Zingiberaceae species with medicinal properties. J. Plant Biotechnology 6 (3) : 181-188.

Chandra R and Govind S (1999). Genetic variability and performance of ginger genotypes under mid-hills of Meghalaya. Indian J. Hort. 56 (3): 274-278.

Chen Z. H., Kai G.Y., Liu X. J., Lin J., Sun X. F. and Tang K. X. (2005). cDNA cloning and characterization of a mannose-binding lectin from Zingiber officinale Roscoe (ginger) rhizomes. Journal of Biosciences 30(2) : 213-220.

Chern, A., Hosskawa Z., Cherubini C. and Cline M. (1993). Effects of node position on lateral bud out growth in the decapitation shoot of Ipomoea nil. J. Sci., 93(1): 11-13.

Chitribul B. and Nalinrut M. (2003). Multiple Shoot Induction and Plant Regeneration of Rosa damascena Mill. Silpakorn Univ. Int. journal, 3(1-2):229.

Cline M. G. (1994). The role of hormones in apical dominance. New approaches to an old problem in plant development. Physiol Plant., 90:230-237.

Cline M. G. (1996). Exogenous auxin effects on lateral bud outgrowth in decapitated shoots. Ann Bot.; 78:255-266.



Copyright © 2023 Author(s) retain the copyright of this article.
This article is published under the terms of the Creative Commons Attribution License 4.0