Distribution of ABO, RH (Rhesus) Blood Grouping and Hepatitis B Among Blood Donors with National Blood Transfusion Service

ABSTRACT

ABO and Rh blood group systems are of major clinical significance amongst the thirty (30) human blood group systems. The Distribution of ABO, Rh blood grouping and hepatitis B virus among blood donors with National Blood Transfusion Service, Kaduna, was carried out. Two hundred voluntary blood donors (200) were used. The questionnaire was used to obtain the respondents’ bio-demographic data. A microplate technique was used for ABO and Rh blood grouping tests, and an ELISA kit (Monolisa HBsAg ULTRA) for the hepatitis B virus test. The results showed that blood group O donors had (47.0%, group A (31.5%), group B (17.0%) and group AB (4.5%). Rh D positive (95.0%) and RhD negative (5%). Reactive hepatitis B (12.5% and non-reactive hepatitis B (87.5%).

CHAPTER ONE

1.0 Introduction

1.1 Aim of the Study

1.2 Objectives of the Study

1.3 Justification of Study

CHAPTER TWO

2.0 REVIEW OF LITERATURE

2.1 Introduction

2.1.1 Screening

2.2 ABO Blood Groups System

2.2.1 ABO blood group genetics

2.2.2 Anti-A and anti-B antibodies

2.2.3 ABO subgroups

2.3 Rhesus (Rh) grouping system

2.3.1 Rh Antigens

2.3.2 Rh Antibodies

2.3.3 Weak Antigen D

2.3.4 Importance of Rh D Grouping

2.4 Hepatitis B Virus (HBV)

2.5 Hepatitis B Surface Antigen

2.6 Hepatitis B Core Antibody

2.7 Hepatitis B Virus DNA

2.8 Microplate technique grouping

2.9 Principle of the monolisaTM HBsAg ULTRA

2.10 Interpretation

2.11 Interpretation for Monolisa HBsAg ULTRA

CHAPTER THREE

3.0 MATERIALS AND METHOD

CHAPTER FOUR

RESULTS

4.1 DISCUSSION

CHAPTER FIVE

CONCLUSION, RECOMMENDATIONS AND LIMITATIONS

5.1 CONCLUSION

5.2 RECOMMENDATIONS

5.3 LIMITATIONS

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